pbluescript (pbs) iiks Search Results


supercoiled pbluescript iiks – (pbs)  (Qiagen)
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Qiagen supercoiled pbluescript iiks – (pbs)
Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or <t>supercoiled</t> circular <t>pBS</t> (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.
Supercoiled Pbluescript Iiks – (Pbs), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or supercoiled circular pBS (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.

Journal:

Article Title: RNA helicase A interacts with dsDNA and topoisomerase II?

doi:

Figure Lengend Snippet: Binding of RHA to circular DNA. (A) Increasing amounts (0.25, 0.5 and 1 µg) of RHA were incubated for 30 min at 37°C in reactions (20 µl) containing 0.25 µg of either linear (lanes 2 and 6–8) or supercoiled circular pBS (lanes 1 and 3–5). Subsequently, reaction mixtures were resolved on a 0.7% agarose gel in 1× TAE, and DNA was visualized by ethidium bromide staining. (B) Increasing amounts (0.125, 0.25, 0.5, 1 and 2 µg) of RHA were added in reactions containing 0.25 µg of supercoiled pBS and 8 ng of topoisomerase IIα. After a 30 min incubation at 37°C, topoisomers were resolved on a 0.7% agarose gel. (C) RHA (1 µg) and topoisomerase IIα (8 ng) or calf thymus topoisomerase I (1 U) were added in the indicated reaction containing 0.25 µg of supercoiled pBS. After a 30 min incubation at 37°C, topoisomers were analyzed as described in (B) and quantified using the QuantityOne software. scDNA, supercoiled DNA.

Article Snippet: Supercoiled pBluescript IIKS – (pBS) was prepared using the Qiagen plasmid kit.

Techniques: Binding Assay, Incubation, Agarose Gel Electrophoresis, Staining, Software